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Research

The eukaryotic endomembrane system is responsible for delivery of newly synthesized protein and lipid to the outside of the cell; for uptake, processing and degradation of extracellular molecules; and for the regeneration and functioning of intracellular organelles. It is comprised of distinct membrane-bound compartments, including ER, Golgi apparatus, endosomes, lysosomes and plasma membrane, among which transport intermediates carry protein and lipid molecules. Within this system, molecules undergo dynamic movements, including lateral diffusion between distinct membrane domains, membrane binding and dissociation into the cytoplasm, and active transport within membrane-bound carriers. Studies in our group are aimed at describing this dynamic activity and relating it to the molecular mechanisms underlying endomembrane structure and function. As this requires methods for visualizing the movement of molecules within cells and for quantifying this movement, a major effort of our lab also has been directed at developing quantitative tools and methods for analyzing protein and organelle dynamics in living cells.

Click on the images below for a brief description of our research.

Endomembrane System

  • IT
    Intercellular Transfer
  • 3D Tubes Link
    Model Membranes
  • 3D Culture Link
    Epithelia in 3D
  • Dynamin 2
    Dynamin in Development
  • Dynamin
    Dynamin in Polarization
  • ER
    Endoplasmic Reticulum
  • Cilia
    Cilia
  • Autophagosome
    Autophagosomes
  • Mitochondria
    Mitochondria
  • Peroxisome
    Peroxisomes
  • Selective Degradation
    Endoplasmic Reticulum Associated Degradation
  • Actin Filament Networks
    Actin Filament Networks
  • Spatial Architecture of Biological Membrane
    Spatial Architecture of Biological Membrane
  • Spatial and temporal organization of Golgi Apparatus
    Spatial and Temporal organization of Golgi Apparatus
  • Stem cell derived germ cells of Drosophila melanogaster
    Stem cell derived germ cells of Drosophila melanogaster
  • Intracellular Mobility of CapG
    Cancer Cell Motility
  • Degradation of Misfolded ER-Proteins
    Degradation of Misfolded ER-Proteins
  • Degradation of Misfolded ER-Proteins
    Dynamin regulates epithelial maintenance and apical constriction in epithelial monolayers

Tools and Methods

  • PALM
    PALM
  • Electron Microscopy
    Electron Microscopy
  • FM
    Fluorescence Microscopy

Movies

  • Dros Movie
    Drosophila ER
  • Golgi Movie
    Golgi
  • Mitochondia Movie
    Mitochondria
  • Peroxisome Movie 2
    Peroxisome (gr) ER (red) nucleus (blue)
  • Peroxisome Movie 1
    Peroxosome (gr) ER (red), zoom
  • GPI Movie
    GPI-GFP trafficking
  • Classic Movies
    Classic Movies